1. 蛋白纯化

• 蛋白表达系统(E.coli, Sf9昆虫细胞,哺乳动物细胞)

• 蛋白标签(His, GST,  FLAG, Avi, MBP, Fc-fusion)

• 蛋白鉴定(SDS Page, Brandford test, LC-MS analysis, Activity test)

 

2.酶学研究

• 结合试验(HTRF, Alpha )， ADP 定量 (ADP-Glo, etc.)

• 磷酸化底物检测 (Ulight, HTRF, Lantha, Alpha)

• 细胞磷酸化蛋白检测(AlphaScreen, HCS, WB)

• 激酶, 表观遗传酶学, E3. DUBs, 磷酸酶, 蛋白酶, 解旋酶, 核酸酶

3.细胞系工程

• 建立了超过1000 株细胞株

• 报告基因，突变, 标签和无标签表达

• 过表达稳转细胞株 (Flip-In, lentiviral and Retroviral system, Neon electroporation, T-REx system, HiBiT and PPI systems etc.)

• 敲出 (KO) 和敲入 (KI) ：CRISPR/Cas 9 system, siRNA, shRNA

4.离子通道

• 基于荧光功能筛选 (FLIPR)

• 电生理实验(手动膜片钳和自动膜片钳QpatchII)

• Panel screening (100+ targets validated)

5.GPCR

• GPCR 稳转细胞系构建

• cAMP(HTRF; agonist/antagonist, CRE-BLA/Fluc)

• 钙流(Flipr; IP-one, NFAT-Luc)

• β-arrestin((PPI/NanoBRET)

• Panel screening (100+ targets validated)

6.核受体

• 荧光偏振结合试验 (lantha)

• 基于TaqMan的基因表达

• 报告基因筛选(transient transfection, stable cell line)

• Panel screening (30+ nuclear receptors)

7.免疫-肿瘤靶点研究

• 检查点阻断生化结合试验和细胞报告基因试验

• 共培养报告基因试验

• 全血, PBMC, T cell, Tregs, DC, B and NK cellular assays

• 体外实验 MLR, co-culture, etc

• 细胞因子释放试验FACS, ELISA

8.Panel screening

• Safety panel (47个/90个靶点)

• 激酶谱 >400 assays validated (provide 207 and 310 panel screening)

• 其他panels: TK family (76x), CDK family (16x) and Mini kinase (60x)

• 定制激酶谱

• 体外脱靶效应筛选(基于不同疾病靶点)

• GLP hERG/心脏安全研究

• Cell panel screening